|
KMID : 1039120130020010058
|
|
Clinical and Experimental Vaccine Research
2013 Volume.2 No. 1 p.58 ~ p.65
|
|
|
Inactivated pep27 mutant as an effective mucosal vaccine against a secondary lethal pneumococcal challenge in mice
|
|
Choi Sang-Yoon
Tran Thao Dang-Hien
Briles David E.
Rhee Dong-Kwon
|
|
|
Abstract
|
|
|
Purpose; A pep27 mutant may be able to elicit mucosal immunity against pneumococcal diseases, and could be employed as an inexpensive attenuated vaccine. However, this particular mutant contains an erythromycin-resistance marker. The purpose of the current study is to develop a markerless pep27 mutant and assess whether this inactivated mutant is able to induce mucosal immunity.
Materials and Methods: Mice were vaccinated intranasally with the inactivated markerless pep27 mutant every 2 weeks for a total of three times, after which time serum samples were analyzed for antibody titers. The mice were then challenged with a lethal D39 strain and their survival time was measured. The cross-reactivity of the antisera against pep27 was also compared to other mutant serotypes.
Results: Intranasal immunization of mice with the inactivated markerless pep27 mutant provides effective protection and rapidly cleared bacterial colonization in vivo. Moreover, antisera raised against the pep27 mutant may cross-react with several other serotype strains.
Conclusion; Intranasal immunization with the inactivated pep27 mutant may be able to provide mucosal immunity, and could represent an efficient mucosal vaccine.
|
|
|
KEYWORD
|
|
|
Streptococcus pneumoniae, pep27 mutant, Inactivated vaccine, No antibiotic resistance marker, Mucosal immunity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|